gerW [2019-06-25 09:35:31]

germination protein

locus: BSU29500

pI: 4.99

mw: 16.20 kDa

protein length: 151 aa

gene length: 456 bp

function: unknown

product: germination protein

essential: no

synonyms: ytfJ

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 3,018,309 3,018,764

Phenotypes of a mutant

• no [SW|germination] in response to l-alanine [Pubmed|23921501], this has been disputed [Pubmed|25790435]

The protein

[SW|Localization]

• in the dormant spore [Pubmed|23921501]

Expression and Regulation

Operons

• genes: [gene|9F7A754B2867CED5CDF3F06009DF5DBDD0877BAF|ytfI]-[gene|1FDD81F50B0B0F260A638FECD9B92F8A5F5ED126|gerW]
  description: [Pubmed|9387221]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|23921501,16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]

Biological materials

Mutant

• MGNA-A112 (ytfJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/112 NBRP B. subtilis, Japan]
• BKE29500 ([gene|1FDD81F50B0B0F260A638FECD9B92F8A5F5ED126|gerW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGGTCTGCCATATACGCTT, downstream forward: _UP4_TAAAAAAAGGTTAACTGCGC
• BKK29500 ([gene|1FDD81F50B0B0F260A638FECD9B92F8A5F5ED126|gerW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGGTCTGCCATATACGCTT, downstream forward: _UP4_TAAAAAAAGGTTAACTGCGC

References

• 16497325,9387221,12107147,23921501,25790435